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psgrnac plasmid  (Addgene inc)


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    Structured Review

    Addgene inc psgrnac plasmid
    Psgrnac Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+psgrnac/psgRNAc+(Plasmid+%23114006)/pmc11416492-51-7-9
    Average 92 stars, based on 4 article reviews
    psgrnac plasmid - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Phages and their satellites encode hotspots of antiviral systems
    Article Snippet: .. A sgRNA targeting hsdR (5’-ATGTTTTCCGGTGGGCCATT-3’) was cloned onto plasmid psgRNAc (Addgene #114006) using the Golden Gate assembly method , giving pFD236, and introduced into the dCas9-expressing E. coli strain ACE1 ( ). ..

    Article Title: Phages and their satellites encode hotspots of antiviral systems
    Article Snippet: .. A sgRNA targeting hsdR (5’-ATGTTTTCCGGTGGGCCATT-3’) was cloned onto plasmid psgRNAc (Addgene #114006) using the Golden Gate assembly method (Engler et al., 2008), giving pFD236, and introduced into the dCas9-expressing E. coli strain ACE1 (Calvo-Villamañán et al., 2020). .. To extract phage DNA, 500 mL of high titer stocks ( 1010 pfu/mL) of wild-type or mutant T7 were treated with TURBO DNAse (Thermo Fisher Scientific) for 30 min at 37 C. DNAse was inactivated with the addition of 5 mL of EDTA 0.5 mM and 25 mL of inactivation reagent at 65 C for 10 min.

    Plasmid Preparation:

    Article Title: Phages and their satellites encode hotspots of antiviral systems
    Article Snippet: .. A sgRNA targeting hsdR (5’-ATGTTTTCCGGTGGGCCATT-3’) was cloned onto plasmid psgRNAc (Addgene #114006) using the Golden Gate assembly method , giving pFD236, and introduced into the dCas9-expressing E. coli strain ACE1 ( ). ..

    Article Title: Phages and their satellites encode hotspots of antiviral systems
    Article Snippet: .. A sgRNA targeting hsdR (5’-ATGTTTTCCGGTGGGCCATT-3’) was cloned onto plasmid psgRNAc (Addgene #114006) using the Golden Gate assembly method (Engler et al., 2008), giving pFD236, and introduced into the dCas9-expressing E. coli strain ACE1 (Calvo-Villamañán et al., 2020). .. To extract phage DNA, 500 mL of high titer stocks ( 1010 pfu/mL) of wild-type or mutant T7 were treated with TURBO DNAse (Thermo Fisher Scientific) for 30 min at 37 C. DNAse was inactivated with the addition of 5 mL of EDTA 0.5 mM and 25 mL of inactivation reagent at 65 C for 10 min.

    Article Title: Phages and their satellites encode hotspots of antiviral systems
    Article Snippet: .. Plasmid psgRNAc , , Addgene Cat #114006. ..



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    Image Search Results


    Journal: Cell Host & Microbe

    Article Title: Phages and their satellites encode hotspots of antiviral systems

    doi: 10.1016/j.chom.2022.02.018

    Figure Lengend Snippet:

    Article Snippet: A sgRNA targeting hsdR (5’-ATGTTTTCCGGTGGGCCATT-3’) was cloned onto plasmid psgRNAc (Addgene #114006) using the Golden Gate assembly method , giving pFD236, and introduced into the dCas9-expressing E. coli strain ACE1 ( ).

    Techniques: Virus, Recombinant, Plasmid Preparation, Software